Preparation method of leech extracting solution

The invention discloses a preparation method of a leech extracting solution. The preparation method comprises the following steps: S4, carrying out reduced pressure suction filtration on a leech intermediate to obtain a suction filtrate and carrying out vacuum heating concentration of the suction filtrate to 1/8-1/3 of the original volume; S5, adding ethanol until the concentration of the ethanol reaches 70%-92%, and carrying out sealing and standing for 20-30 h; and S6, carrying out reduced pressure suction filtration again to obtain a filtrate and concentrating the filtrate to 1/8-1/3 of the original volume. The obtained leech extracting solution has high antithrombin content and high yield, and a extracting process is economical, stable and feasible. 本发明公开了一种水蛭提取液制备方法，其特征是，包括如下步骤：S4:将水蛭中间物减压抽滤后得抽滤液，真空加热浓缩至原体积的1/8～1/3；S5:加入乙醇，使乙醇浓度达到70％～92％，密封，静置20～30h；S6:再次进行减压抽滤，得滤液，浓缩至原体积的1/8～1/3，即可。本发明所得的水蛭提取液抗凝血酶含量高，收率高，提取工艺经济、稳定、可行。