IMMUNOGLOBULIN-BINDING PROTEIN, AND AFFINITY CARRIER USING SAME

Provided is an affinity carrier having improved alkali resistance. This immunoglobulin-binding protein contains a mutant polypeptide chain. This affinity carrier includes a solid-phase carrier to which the immunoglobulin-binding protein is bound. The mutant polypeptide chain has an amino acid sequen...

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Bibliographische Detailangaben
Hauptverfasser:	ONOGI SHUNSUKE, NAKAMURA SATOSHI, HONDA TOSHINARI, KAMIDE TOMOYUKI, HAGA TOMOAKI, ICHII TAKASHI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided is an affinity carrier having improved alkali resistance. This immunoglobulin-binding protein contains a mutant polypeptide chain. This affinity carrier includes a solid-phase carrier to which the immunoglobulin-binding protein is bound. The mutant polypeptide chain has an amino acid sequence having at least 85% identity to an amino acid sequence indicated by any of SEQ ID NO: 1-6 and 57-62 and having a predetermined mutation, and has immunoglobulin-binding activity. 本发明提供耐碱性提升的亲和载体。一种亲和载体，其包含：含有变异多肽链的免疫球蛋白结合蛋白质，和键合该免疫球蛋白结合蛋白质的固相载体。该变异多肽链是由与序列号1～6和57～62中的任一个所示的氨基酸序列具有至少85％同源性且具有规定的变异的氨基酸序列构成，具有免疫球蛋白结合活性。