Specific sequence and kit for identifying Yunyan 87 cured variety and use method of kit

The invention relates to a specific sequence and kit for identifying a Yunyan 87 cured variety and a use method of the kit, and belongs to the field of biomolecular identification. The kit comprises the specific sequence, and primers and probes designed and synthesized according to the specific sequence, the kit further comprises a multichannel ultralow microfluidic nucleic acid detection chip for sensitively and intelligently controlling flow paths, pre-loaded dry powder detection reagents and positive quality control products. During use, different samples are added into sample adding holes of the microfluidic nucleic acid detection chip, the sample adding holes are covered with the sample adding hole covers, and the microfluidic nucleic acid detection chip is placed into a microfluidic chip detector for detection; PCR amplification is carried out, and result judgment is carried out. Compared with a common fluorescent PCR method and the like, the primers and the probes have the following advantage of high s