Determination method for detecting total coliform, fecal coliform and Escherichia coli in water
The invention discloses a determination method for detecting total coliform, fecal coliform and Escherichia coli in water. The determination method comprises the following steps that step 1, reagents and materials are prepared; step 2, instruments and equipment are prepared; step 3, samples are coll...
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| creator | ZHOU YONGFANG XIA HAILONG ZHAN ZIKUAN SUN SHUAI LIN KONGLIANG WU SUHUI |
| description | The invention discloses a determination method for detecting total coliform, fecal coliform and Escherichia coli in water. The determination method comprises the following steps that step 1, reagents and materials are prepared; step 2, instruments and equipment are prepared; step 3, samples are collected; step 4, the samples are determinate and tested; and step 5, a test result is calculated and represented. The reagents and materials prepared in the step 1 is prepared from the materials that (1), a culture medium, specifically, a Minimum Medium ONPG-MUG culture medium is adopted, and 2.7 g +/-0.5 g of culture medium powder needs to be used for every 100 ml of sample; (2), sodium thiosulfate (Na2S2O3.5H2O) is adopted; (3), ethylenediamine tetraacetic acid disodium salt (C10H14N2O8Na2.2H2O) is adopted; and (4), sodium thiosulfate solution is adopted, rho(Na2S2O3) is 0.10 g/ml (15.7 g of sodium thiosulfate is weighed and dissolved in a proper amount of water, the volume is fixed to 100 ml, and the sodium thiosu |
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The determination method comprises the following steps that step 1, reagents and materials are prepared; step 2, instruments and equipment are prepared; step 3, samples are collected; step 4, the samples are determinate and tested; and step 5, a test result is calculated and represented. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Determination method for detecting total coliform, fecal coliform and Escherichia coli in water |
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