Determination method for detecting total coliform, fecal coliform and Escherichia coli in water

The invention discloses a determination method for detecting total coliform, fecal coliform and Escherichia coli in water. The determination method comprises the following steps that step 1, reagents and materials are prepared; step 2, instruments and equipment are prepared; step 3, samples are collected; step 4, the samples are determinate and tested; and step 5, a test result is calculated and represented. The reagents and materials prepared in the step 1 is prepared from the materials that (1), a culture medium, specifically, a Minimum Medium ONPG-MUG culture medium is adopted, and 2.7 g +/-0.5 g of culture medium powder needs to be used for every 100 ml of sample; (2), sodium thiosulfate (Na2S2O3.5H2O) is adopted; (3), ethylenediamine tetraacetic acid disodium salt (C10H14N2O8Na2.2H2O) is adopted; and (4), sodium thiosulfate solution is adopted, rho(Na2S2O3) is 0.10 g/ml (15.7 g of sodium thiosulfate is weighed and dissolved in a proper amount of water, the volume is fixed to 100 ml, and the sodium thiosu