Non-denaturing purification method and application of soluble recombinant teriparatide
The invention discloses a non-denaturing purification method and application of soluble recombinant teriparatide. The non-denaturing purification method comprises the following steps: 1) thallus cracking; 2) nickel column affinity purification; 3) enzyme digestion; 4) nickel column penetration; (5)...
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| Format: | Patent |
| Sprache: | chi ; eng |
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| Zusammenfassung: | The invention discloses a non-denaturing purification method and application of soluble recombinant teriparatide. The non-denaturing purification method comprises the following steps: 1) thallus cracking; 2) nickel column affinity purification; 3) enzyme digestion; 4) nickel column penetration; (5) purifying by using a C18-100A column; (6) C18-300A reverse chromatography purification; and 7) rotary evaporation and freeze-drying. According to the non-denaturing purification method and application of the soluble recombinant teriparatide, Trx-hPTH(1-34) fusion protein is separated from an expression product by a nickel affinity chromatography method, then the recombinant teriparatide is released from the Trx-hPTH(1-34) fusion protein by enzyme digestion, and the purified recombinant teriparatide is prepared by a three-step chromatography method, so that the purification process is simplified, the purification efficiency and purity of the soluble recombinant teriparatide are improved, and important help is provid |
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