Lipase mutant D170A with improved catalytic activity and application thereof

The divisional application discloses a lipase mutant D170A with improved catalytic activity and application thereof. The amino acid sequence of the lipase mutant D170A is shown as SEQ ID NO.4, the lipase mutant D170A is obtained by changing the 170th aspartic acid in the amino acid sequence SEQ ID N...

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Bibliographische Detailangaben
Hauptverfasser:	HUANG ZUNXI, JIANG ZHANBAO, HAN NANYU, TANG MINYUAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FODDER FOODS OR FOODSTUFFS HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	The divisional application discloses a lipase mutant D170A with improved catalytic activity and application thereof. The amino acid sequence of the lipase mutant D170A is shown as SEQ ID NO.4, the lipase mutant D170A is obtained by changing the 170th aspartic acid in the amino acid sequence SEQ ID NO.1 of thermomyces lanuginosus lipase into alanine, and the coding gene of the lipase mutant D170A is shown as SEQ ID NO.8. The catalytic activity of the mutant is improved. 本分案申请公开了一种催化活性提高的脂肪酶突变体D170A及其应用，所述的脂肪酶突变体D170A的氨基酸序列如SEQ ID NO.4所示，脂肪酶突变体D170A由疏棉状嗜热丝孢菌脂肪酶氨基酸序列SEQ ID NO.1中第170位天冬氨酸变为丙氨酸而获得，其编码基因如SEQ ID NO.8所示。该突变体的催化活性提高。