Preparation method of yeast with high acid resistance

The invention discloses a preparation method of saccharomycetes with high acid resistance. The preparation method comprises the following steps: steps 1, strain activation; step 2, strain screening; step 3, strain culture; and step 4, strain screening. During strain activation in the step 1, a strain is placed in a sterilized YPD liquid triangular flask culture medium and subjected to shaking culture for 20 hours to 26 hours at a temperature of 24 DEG C and 28 DEG C with the rotating speed of a shaking table being 230 rpm. During strain screening in the step 2, a proper amount of enriched saccharomycete communities is diluted in a wort culture medium and subjected strain observation under a microscope, the growth conditions of the strains are observed, a single colony is selected, and streak separation and screening are conducted. According to the preparation method of the high-acid-resistance saccharomycetes, the acid-resistance saccharomycetes can be rapidly prepared, the quality of the saccharomycetes is g