Construction method of SARS-CoV-2-caused gastroenteritis animal model
The invention belongs to the technical field of animal model construction, and discloses a construction method of a gastroenteritis animal model caused by SARS-CoV-2. The method comprises the following steps: before simulating virus infection, carrying out acid enema treatment on gastrointestinal tr...
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| creator | HE JIANZHONG DENG ZHAOHUA ZENG FAMIN HE HUANHUAN LI YINGWEN |
| description | The invention belongs to the technical field of animal model construction, and discloses a construction method of a gastroenteritis animal model caused by SARS-CoV-2. The method comprises the following steps: before simulating virus infection, carrying out acid enema treatment on gastrointestinal tract epithelium, adding acetic acid to cause a slight injury, and after carrying out acid enema treatment, intraperitoneally injecting SARS-CoV-2 Spike Protein to facilitate the reproduction of the simulated mouse viral gastrointestinal tract symptoms; the animal model obtained through the method is consistent with clinical symptoms of COVID-19 patients reported in the prior art, so that the animal model constructed through the method can copy gastrointestinal tract manifestations of the clinical COVID-19 patients.
本发明属于动物模型构建技术领域，公开了SARS-CoV-2引起的胃肠炎症动物模型的构建方法，是在模拟病毒感染之前，先对胃肠道上皮进行酸灌肠处理，添加的乙酸能够造成一个轻微的损伤，在进行酸灌肠处理后，再腹腔注射SARS-CoV-2 Spike Protein更有利于模拟小鼠病毒性胃肠道症状的重现；经过这种方式获得的动物模型与现有技术报道的有关COVID-19患者的临床症状一致，因此本发明方法构建的动物模型可 |
| format | Patent |
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本发明属于动物模型构建技术领域，公开了SARS-CoV-2引起的胃肠炎症动物模型的构建方法，是在模拟病毒感染之前，先对胃肠道上皮进行酸灌肠处理，添加的乙酸能够造成一个轻微的损伤，在进行酸灌肠处理后，再腹腔注射SARS-CoV-2 Spike Protein更有利于模拟小鼠病毒性胃肠道症状的重现；经过这种方式获得的动物模型与现有技术报道的有关COVID-19患者的临床症状一致，因此本发明方法构建的动物模型可</description><language>chi ; eng</language><subject>HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210406&DB=EPODOC&CC=CN&NR=112603581A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210406&DB=EPODOC&CC=CN&NR=112603581A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HE JIANZHONG</creatorcontrib><creatorcontrib>DENG ZHAOHUA</creatorcontrib><creatorcontrib>ZENG FAMIN</creatorcontrib><creatorcontrib>HE HUANHUAN</creatorcontrib><creatorcontrib>LI YINGWEN</creatorcontrib><title>Construction method of SARS-CoV-2-caused gastroenteritis animal model</title><description>The invention belongs to the technical field of animal model construction, and discloses a construction method of a gastroenteritis animal model caused by SARS-CoV-2. The method comprises the following steps: before simulating virus infection, carrying out acid enema treatment on gastrointestinal tract epithelium, adding acetic acid to cause a slight injury, and after carrying out acid enema treatment, intraperitoneally injecting SARS-CoV-2 Spike Protein to facilitate the reproduction of the simulated mouse viral gastrointestinal tract symptoms; the animal model obtained through the method is consistent with clinical symptoms of COVID-19 patients reported in the prior art, so that the animal model constructed through the method can copy gastrointestinal tract manifestations of the clinical COVID-19 patients.
本发明属于动物模型构建技术领域，公开了SARS-CoV-2引起的胃肠炎症动物模型的构建方法，是在模拟病毒感染之前，先对胃肠道上皮进行酸灌肠处理，添加的乙酸能够造成一个轻微的损伤，在进行酸灌肠处理后，再腹腔注射SARS-CoV-2 Spike Protein更有利于模拟小鼠病毒性胃肠道症状的重现；经过这种方式获得的动物模型与现有技术报道的有关COVID-19患者的临床症状一致，因此本发明方法构建的动物模型可</description><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZHB1zs8rLikqTS7JzM9TyE0tychPUchPUwh2DArWdc4P0zXSTU4sLU5NUUhPBKrLT80rSS3KLMksVkjMy8xNzFHIzU9JzeFhYE1LzClO5YXS3AyKbq4hzh66qQX58anFBYnJqXmpJfHOfoaGRmYGxqYWho7GxKgBAKsiMpw</recordid><startdate>20210406</startdate><enddate>20210406</enddate><creator>HE JIANZHONG</creator><creator>DENG ZHAOHUA</creator><creator>ZENG FAMIN</creator><creator>HE HUANHUAN</creator><creator>LI YINGWEN</creator><scope>EVB</scope></search><sort><creationdate>20210406</creationdate><title>Construction method of SARS-CoV-2-caused gastroenteritis animal model</title><author>HE JIANZHONG ; DENG ZHAOHUA ; ZENG FAMIN ; HE HUANHUAN ; LI YINGWEN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN112603581A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2021</creationdate><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS</topic><toplevel>online_resources</toplevel><creatorcontrib>HE JIANZHONG</creatorcontrib><creatorcontrib>DENG ZHAOHUA</creatorcontrib><creatorcontrib>ZENG FAMIN</creatorcontrib><creatorcontrib>HE HUANHUAN</creatorcontrib><creatorcontrib>LI YINGWEN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HE JIANZHONG</au><au>DENG ZHAOHUA</au><au>ZENG FAMIN</au><au>HE HUANHUAN</au><au>LI YINGWEN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Construction method of SARS-CoV-2-caused gastroenteritis animal model</title><date>2021-04-06</date><risdate>2021</risdate><abstract>The invention belongs to the technical field of animal model construction, and discloses a construction method of a gastroenteritis animal model caused by SARS-CoV-2. The method comprises the following steps: before simulating virus infection, carrying out acid enema treatment on gastrointestinal tract epithelium, adding acetic acid to cause a slight injury, and after carrying out acid enema treatment, intraperitoneally injecting SARS-CoV-2 Spike Protein to facilitate the reproduction of the simulated mouse viral gastrointestinal tract symptoms; the animal model obtained through the method is consistent with clinical symptoms of COVID-19 patients reported in the prior art, so that the animal model constructed through the method can copy gastrointestinal tract manifestations of the clinical COVID-19 patients.
本发明属于动物模型构建技术领域，公开了SARS-CoV-2引起的胃肠炎症动物模型的构建方法，是在模拟病毒感染之前，先对胃肠道上皮进行酸灌肠处理，添加的乙酸能够造成一个轻微的损伤，在进行酸灌肠处理后，再腹腔注射SARS-CoV-2 Spike Protein更有利于模拟小鼠病毒性胃肠道症状的重现；经过这种方式获得的动物模型与现有技术报道的有关COVID-19患者的临床症状一致，因此本发明方法构建的动物模型可</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS |
| title | Construction method of SARS-CoV-2-caused gastroenteritis animal model |
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