Construction method of SARS-CoV-2-caused gastroenteritis animal model

The invention belongs to the technical field of animal model construction, and discloses a construction method of a gastroenteritis animal model caused by SARS-CoV-2. The method comprises the following steps: before simulating virus infection, carrying out acid enema treatment on gastrointestinal tract epithelium, adding acetic acid to cause a slight injury, and after carrying out acid enema treatment, intraperitoneally injecting SARS-CoV-2 Spike Protein to facilitate the reproduction of the simulated mouse viral gastrointestinal tract symptoms; the animal model obtained through the method is consistent with clinical symptoms of COVID-19 patients reported in the prior art, so that the animal model constructed through the method can copy gastrointestinal tract manifestations of the clinical COVID-19 patients. 本发明属于动物模型构建技术领域，公开了SARS-CoV-2引起的胃肠炎症动物模型的构建方法，是在模拟病毒感染之前，先对胃肠道上皮进行酸灌肠处理，添加的乙酸能够造成一个轻微的损伤，在进行酸灌肠处理后，再腹腔注射SARS-CoV-2 Spike Protein更有利于模拟小鼠病毒性胃肠道症状的重现；经过这种方式获得的动物模型与现有技术报道的有关COVID-19患者的临床症状一致，因此本发明方法构建的动物模型可