Designing artificial base sequences for binding to polynucleic acid biomarkers and methods of using polynucleic acid probes of artificial base sequences

The present invention relates to a polynucleic acid probe comprising a mononucleotide sequence that binds to a biomarker for the detection of a plurality of nucleic acids. The artificial nucleotide sequence designed by the method for designing the artificial nucleotide sequence shows better hybridization reactivity with all simulants, and the multi-nucleic-acid probe containing the artificial nucleotide sequence is designed to enable a single diagnostic probe to be combined with multiple types of meaningful nucleic acids at the same time so as to detect an overall expression product in the sample. Accordingly, the present application uses the single diagnostic probe to achieve ultra-multiplex diagnosis of nucleic acid biomarkers, thereby improving diagnostic capability and significantly reducing examination costs. 本发明涉及包含单核苷酸序列的多核酸探针，所述单核苷酸序列与用于检测多种核酸的生物标志物结合。通过本发明所述的设计人工核苷酸序列的方法设计的人工核苷酸序列显示出与所有模拟物更好的杂交反应性，并且包含人工核苷酸序列的多核酸探针被设计成使得单个诊断探针能够同时结合多种类型的具有意义的核酸，以检测样品中的整体表达产物。相应地，本申请使用单个诊断探针可实现核酸生物标志物的超多重诊断，由此可以提高诊断能力