Method for obtaining male individual sperm cells from seminal fluid vaginal fluid mixed spots

The invention belongs to the technical field of forensic medicine, and relates to a method for separating cells by immunomagnetic beads, in particular to a method for separating male individual spermcells from seminal fluid vaginal fluid mixed spots by utilizing an immunomagnetic beads technology. The method comprises the following steps: step 1, mixing male seminal fluid and female vaginal fluidaccording to the principle of informed consent; step 2, coupling the sperms by using a biotin labeled ACRBP antibody, and enriching and collecting sperm cells of a male individual by using avidin coupled magnetic beads and an external magnetic field. and step 3, carrying out PCR amplification and capillary electrophoresis so as to perform genotyping on the STR locus. The method provided by the invention can effectively determine the source of the individual DNA in the male individual seminal stain from the gene level, greatly simplify the step of identifying the tissue source of the body fluid or its spots at the crim