CH3 DOMAIN EPITOPE TAGS

This invention relates to the incorporation of one, or more, heterologous antibody epitopes into the AB, EF, or CD structural loops of the constant heavy domain 3 (''CH3 domain'') of an engineered antibody or Fc-linked therapeutic agent. The heterologous epitopes serve as "e...

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Bibliographische Detailangaben
Hauptverfasser:	MORIN MICHAEL J, ROBINSON MATTHEW K
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	This invention relates to the incorporation of one, or more, heterologous antibody epitopes into the AB, EF, or CD structural loops of the constant heavy domain 3 (''CH3 domain'') of an engineered antibody or Fc-linked therapeutic agent. The heterologous epitopes serve as "epitope tags" that are specifically detectable by epitope tag-specific detector antibodies, irrespective of the tagged agent'starget specificity. Therefore, the epitope tags are useful for the rapid detection of any tagged antibody or Fc- linked agent in biological samples, including samples, which also contain endogenous antibodies. 本发明涉及将一种或多种异源抗体表位掺入工程化抗体或Fc连接的治疗剂的恒定重链结构域3("CH3结构域")的AB、EF或CD结构环中。异源表位充当"表位标签"，其可以由表位标签特异性检测抗体特异性检测，而与标记药剂的靶标特异性无关。因此，表位标签可用于快速检测生物样品(包括还含有内源性抗体的样品)中任何标记的抗体或Fc连接药剂。