CALIBRATION OF MULTISPECTRAL ANALYSIS SYSTEMS

Methods for calibrating a multispectral analysis system (1000) include calibrating the system to detect fluorescence emission from a first fluorescent entity in a biological sample (900) that includesthe first fluorescent entity and a second fluorescent entity using a calibration sample, where the calibration sample features a first concentration of the first fluorescent entity and a second concentration of the second fluorescent entity, and where the first concentration is larger than the second concentration. 用于校准多光谱分析系统(1000)的方法包括校准该系统以使用校准样品检测来自包括第一荧光实体和第二荧光实体的生物样品(900)中的第一荧光实体的荧光发射，其中校准样品的特征在于第一浓度的第一荧光实体和第二浓度的第二荧光实体，并且其中第一浓度大于第二浓度。