Efficient genome editing vector based on CRISPR-SaCas9 system and application of efficient genome editing vector

The invention provides an efficient genome editing vector based on a CRISPR-SaCas9 system and application of the efficient genome editing vector, and belongs to the field of plant genetic engineering.The invention also provides a method for introducing the efficient genome editing vector into rice cells. The efficient genome editing vector comprises a first expression cassette and an optional second expression cassette, wherein the first expression cassette is an sgRNA expression cassette, and the second expression cassette is an SaCas9-NLS fusion protein expression cassette. By utilizing thegene editing vector disclosed by the invention, gene mutation can be simply, conveniently and efficiently carried out at a predetermined rice genome site. 本发明提供了一种基于CRISPR-SaCas9系统的高效基因组编辑载体及其应用，属于植物基因工程领域。本发明还提供了将其导入水稻细胞的方法。本发明的高效基因组编辑载体包括第一表达盒和任选的第二表达盒，所述的第一表达盒为sgRNA表达盒，第二表达盒为SaCas9-NLS融合蛋白表达盒。利用本发明的基因编辑载体，可以在预定的水稻基因组位点，简便而高效地进行基因突变。