Tissue culture method of red-root wild broad beans

The invention provides a tissue culture method of red-root wild broad beans. The tissue culture method comprises seed surface disinfection and sterile sowing measures, axillary bud induction and multiplication culture measures, rooting induction culture measures and measures for influencing rooting and seedling strengthening by additives. Through the method provided by the invention, more propagules can be obtained through rapid propagation, the defects of less rooting and difficult rooting of the red-root wild broad beans under natural conditions are overcome, and the survival rate of cultivation and domestication is improved. 本发明提供了一种红根野蚕豆组织培养方法，包括种子表面消毒与无菌播种措施、腋芽诱导及其增殖培养措施、生根诱导培养措施、添加物对生根壮苗的影响措施。通过本发明提供的方法，可以快速繁殖得到更多繁殖体、克服了红根野蚕豆自然条件下生根少、生根困难的缺点，提高了栽培驯化成活率。