Recombinant yarrowia lipolytica strain with high oleic acid yield as well as construction method and application thereof

The invention provides recombinant yarrowia lipolytica with high oleic acid yield as well as a construction method and application thereof, and belongs to the field of bioengineering. The recombinantyarrowia lipolytica is a yarrowia lipolytica XJ-9 strain, is deposited in the China Center for Type Culture Collection (CCTCC), and has a deposit number of CCTCC NO: M 2020707. According to the yarrowia lipolytica XJ-9 strain, oleate desaturase is knocked out, endogenous acetyl coenzyme A carboxylase, diacylglycerol acyltransferase and fatty acid elongase 1 are overexpressed, heterologous fatty acid elongase 2 and stearoyl coenzyme A desaturase are overexpressed, and experiments prove that the recombinant yarrowia lipolytica can be efficiently fermented to produce oleic acid, and efficient synthesis of plant-derived natural product oleic acid in yarrowia lipolytica is realized. 本发明提供一株高产油酸的重组解脂耶氏酵母菌及其构建方法和应用，属于生物工程领域。所述高产油酸的重组解脂耶氏酵母菌，是解脂耶氏酵母（Yarrowia lipolytica）XJ-9株，已在中国典型培养物保藏中心保藏，保藏编号为CCTCC NO：M 2020707。本发明解脂耶氏