Method for cultivating broad-spectrum bacterial leaf streak resistant rice, primer and expression cassette
The invention discloses a method for cultivating broad-spectrum bacterial leaf streak resistant rice, a primer and an expression cassette. The method comprises the following step that an OsSULTR3;6 gene promoter region is edited, so target sequences from the 422th site to the 403rd site on the upstr...
Gespeichert in:
| Hauptverfasser: | , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | HUANG SHENG JIN XIA LIAO ZHOUXIANG NI ZHE |
| description | The invention discloses a method for cultivating broad-spectrum bacterial leaf streak resistant rice, a primer and an expression cassette. The method comprises the following step that an OsSULTR3;6 gene promoter region is edited, so target sequences from the 422th site to the 403rd site on the upstream of a transcription start site of the OsSULTR3;6 gene are mutated to obtain the broad-spectrum bacterial leaf streak resistant rice; and the target sequences are nucleotide sequences as shown in SEQ ID No. 1. The provided method proposes constructing the bacterial leaf streak resistant rice through gene editing and breeding for the first time. According to the method, the OsSULTR3;6 gene promoter region is edited based on a CRISPR/Cas9 system; the obtained disease-resistant rice has no transgenic marker, the cultured new variety is similar to a natural mutation result, and the method can be widely applied to resistance breeding of various susceptible rice, is not limited by rice varieties, directionally changes |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN112522300A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN112522300A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN112522300A3</originalsourceid><addsrcrecordid>eNqNyjEOgkAQRmEaC6PeYewlAYwHMERjo5U9GZYfXYXdzcxgPL4WHsDqFd-bZ48z7B476qOQmwbzLzYfbtRK5C7XBGcyjdSyM4jngQZwT2oCfpJAvRoHI_EOG0riRwhx6Ajv9FX1MZBjVZhhmc16HhSrXxfZ-ni41qccKTbQxA4B1tSXsqx2VbUtiv32n-cD4dxBjg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Method for cultivating broad-spectrum bacterial leaf streak resistant rice, primer and expression cassette</title><source>esp@cenet</source><creator>HUANG SHENG ; JIN XIA ; LIAO ZHOUXIANG ; NI ZHE</creator><creatorcontrib>HUANG SHENG ; JIN XIA ; LIAO ZHOUXIANG ; NI ZHE</creatorcontrib><description>The invention discloses a method for cultivating broad-spectrum bacterial leaf streak resistant rice, a primer and an expression cassette. The method comprises the following step that an OsSULTR3;6 gene promoter region is edited, so target sequences from the 422th site to the 403rd site on the upstream of a transcription start site of the OsSULTR3;6 gene are mutated to obtain the broad-spectrum bacterial leaf streak resistant rice; and the target sequences are nucleotide sequences as shown in SEQ ID No. 1. The provided method proposes constructing the bacterial leaf streak resistant rice through gene editing and breeding for the first time. According to the method, the OsSULTR3;6 gene promoter region is edited based on a CRISPR/Cas9 system; the obtained disease-resistant rice has no transgenic marker, the cultured new variety is similar to a natural mutation result, and the method can be widely applied to resistance breeding of various susceptible rice, is not limited by rice varieties, directionally changes</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TRAPPING ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210319&DB=EPODOC&CC=CN&NR=112522300A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210319&DB=EPODOC&CC=CN&NR=112522300A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HUANG SHENG</creatorcontrib><creatorcontrib>JIN XIA</creatorcontrib><creatorcontrib>LIAO ZHOUXIANG</creatorcontrib><creatorcontrib>NI ZHE</creatorcontrib><title>Method for cultivating broad-spectrum bacterial leaf streak resistant rice, primer and expression cassette</title><description>The invention discloses a method for cultivating broad-spectrum bacterial leaf streak resistant rice, a primer and an expression cassette. The method comprises the following step that an OsSULTR3;6 gene promoter region is edited, so target sequences from the 422th site to the 403rd site on the upstream of a transcription start site of the OsSULTR3;6 gene are mutated to obtain the broad-spectrum bacterial leaf streak resistant rice; and the target sequences are nucleotide sequences as shown in SEQ ID No. 1. The provided method proposes constructing the bacterial leaf streak resistant rice through gene editing and breeding for the first time. According to the method, the OsSULTR3;6 gene promoter region is edited based on a CRISPR/Cas9 system; the obtained disease-resistant rice has no transgenic marker, the cultured new variety is similar to a natural mutation result, and the method can be widely applied to resistance breeding of various susceptible rice, is not limited by rice varieties, directionally changes</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TRAPPING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyjEOgkAQRmEaC6PeYewlAYwHMERjo5U9GZYfXYXdzcxgPL4WHsDqFd-bZ48z7B476qOQmwbzLzYfbtRK5C7XBGcyjdSyM4jngQZwT2oCfpJAvRoHI_EOG0riRwhx6Ajv9FX1MZBjVZhhmc16HhSrXxfZ-ni41qccKTbQxA4B1tSXsqx2VbUtiv32n-cD4dxBjg</recordid><startdate>20210319</startdate><enddate>20210319</enddate><creator>HUANG SHENG</creator><creator>JIN XIA</creator><creator>LIAO ZHOUXIANG</creator><creator>NI ZHE</creator><scope>EVB</scope></search><sort><creationdate>20210319</creationdate><title>Method for cultivating broad-spectrum bacterial leaf streak resistant rice, primer and expression cassette</title><author>HUANG SHENG ; JIN XIA ; LIAO ZHOUXIANG ; NI ZHE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN112522300A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2021</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TRAPPING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>HUANG SHENG</creatorcontrib><creatorcontrib>JIN XIA</creatorcontrib><creatorcontrib>LIAO ZHOUXIANG</creatorcontrib><creatorcontrib>NI ZHE</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HUANG SHENG</au><au>JIN XIA</au><au>LIAO ZHOUXIANG</au><au>NI ZHE</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for cultivating broad-spectrum bacterial leaf streak resistant rice, primer and expression cassette</title><date>2021-03-19</date><risdate>2021</risdate><abstract>The invention discloses a method for cultivating broad-spectrum bacterial leaf streak resistant rice, a primer and an expression cassette. The method comprises the following step that an OsSULTR3;6 gene promoter region is edited, so target sequences from the 422th site to the 403rd site on the upstream of a transcription start site of the OsSULTR3;6 gene are mutated to obtain the broad-spectrum bacterial leaf streak resistant rice; and the target sequences are nucleotide sequences as shown in SEQ ID No. 1. The provided method proposes constructing the bacterial leaf streak resistant rice through gene editing and breeding for the first time. According to the method, the OsSULTR3;6 gene promoter region is edited based on a CRISPR/Cas9 system; the obtained disease-resistant rice has no transgenic marker, the cultured new variety is similar to a natural mutation result, and the method can be widely applied to resistance breeding of various susceptible rice, is not limited by rice varieties, directionally changes</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN112522300A |
| source | esp@cenet |
| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE |
| title | Method for cultivating broad-spectrum bacterial leaf streak resistant rice, primer and expression cassette |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T08%3A13%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=HUANG%20SHENG&rft.date=2021-03-19&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN112522300A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |