Method for cultivating broad-spectrum bacterial leaf streak resistant rice, primer and expression cassette

The invention discloses a method for cultivating broad-spectrum bacterial leaf streak resistant rice, a primer and an expression cassette. The method comprises the following step that an OsSULTR3;6 gene promoter region is edited, so target sequences from the 422th site to the 403rd site on the upstream of a transcription start site of the OsSULTR3;6 gene are mutated to obtain the broad-spectrum bacterial leaf streak resistant rice; and the target sequences are nucleotide sequences as shown in SEQ ID No. 1. The provided method proposes constructing the bacterial leaf streak resistant rice through gene editing and breeding for the first time. According to the method, the OsSULTR3;6 gene promoter region is edited based on a CRISPR/Cas9 system; the obtained disease-resistant rice has no transgenic marker, the cultured new variety is similar to a natural mutation result, and the method can be widely applied to resistance breeding of various susceptible rice, is not limited by rice varieties, directionally changes