Preparation and application of myoglobin monoclonal antibody

The invention belongs to the technical field of biology. The invention provides a recombinant protein. An amino acid sequence of the recombinant protein is formed by repeatedly connecting two dominantepitopes of myoglobin (Myo) in series, an escherichia coli preferred codon is adopted to convert the amino acid sequence of the recombinant protein into a corresponding nucleotide sequence, the nucleotide sequence is chemically synthesized, and a recombinant expression vector is constructed. Therefore, the expression quantity of the recombinant protein in escherichia coli is increased. The invention also relates to a phage library established by immunizing mice with the recombinant protein, a corresponding myoglobin single-chain antibody scfv sequence is obtained through selecting and screening, the obtained scfv sequence is constructed into a complete mouse IgG1 antibody sequence expression vector, a monoclonal antibody is expressed through transient HEK293F cells, the monoclonal antibody is purified, and an opt