Preparation method of bovine serum albumin

The invention relates to the technical field of biology, in particular to a preparation method of bovine serum albumin. The preparation method of BSA provided by the invention comprises the followingsteps: serum dilution, first-step extraction, second-step extraction, and washing, filtering and concentration. Experiments show that by adopting the method provided by the invention, a good extraction effect can be obtained under proper parameters, the yield is relatively high, the batch-to-batch difference is small, the chemical residue is reduced, the anti-interference capability is improved, and the non-specific adsorption of a detection reagent can be remarkably reduced by using the obtained product as a key auxiliary material protein of the detection reagent. 本发明涉及生物技术领域，尤其涉及牛血清白蛋白的制备方法。本发明提供的BSA的制备方法中，包括：血清稀释、第一步提取、第二步提取、洗滤浓缩几个步骤。实验表明，采用本发明提供的方法在适宜的参数下，能够获得良好的提取效果，不仅收率较高，并且批间差异小，降低了化学残留，提高了抗干扰能力，所得产品作为检测试剂的关键辅料蛋白，能够显著降低检测试剂的非特异性吸附。