Two sgRNA guide sequences specifically targeting pig Pax7 gene and application

The invention belongs to the technical field of animal gene engineering, and particularly relates to two sgRNA guide sequences specifically targeting a pig Pax7 genes and an application. According tothe invention, four sgRNA guide sequences are designed near an initiation codon ATG of the pig Pax7 gene, and two sgRNAs capable of specifically targeting the vicinity of the initiation codon ATG of the pig Pax7 gene are selected from the four sgRNA guide sequences by using a CRISPR/Cas9 system. By combining the sgRNA sequences provided by the invention with the CRISPR/Cas9 system for use, editingor knockout of the Pax7 gene in pigs can be realized, so that marking or low expression of the Pax7 gene is realized, and a technical foundation is laid for preparation of Pax7 transgenic pigs. 本发明属于动物基因工程技术领域，具体涉及两条特异性靶向猪Pax7基因的sgRNA导向序列及应用。本发明在猪Pax7基因起始密码子ATG附近位置设计了4个sgRNA导向序列，利用CRISPR/Cas9系统，从中挑选出能够特异性靶向猪Pax7基因起始密码子ATG附近的两条sgRNA。通过将本发明提供的sgRNA序列以及CRISPR/Cas9系统的结合起来使用，可以实现猪中Pax7基因的编辑或敲除，进而实现Pax7基因的标记或低表达，为制备Pax7转基因猪奠定了技