COLUMN HARDWARE AND SEPARATION COLUMN, AND METHOD FOR MANUFACTURING SAME

Disclosed is a column hardware (1) for liquid chromatography, one of the purposes of which is to prevent non-specific metal coordination adsorption of a solute in a sample and to prevent non-specificadsorption of an alkaline solute and non-specific hydrophobic adsorption of a highly hydrophobic solute while maintaining high pressure resistance using a metal column hardware column. As one embodiment for this purpose, a first film (41) having SiO2 as the main component is laminated on a metal mobile phase liquid contact part (3) of a column hardware (1) for liquid chromatography, and a second film (42) obtained by silanization of silanol groups on the surface of SiO2 is laminated on the surface (411) of the first film (41). 本发明公开了一种液相色谱法用柱硬件(1)，其目的之一在于，在使用金属制柱硬件柱而设为高耐压的同时，防止试样中的溶质的非特异性金属配位吸附，并且防止碱性的溶质的非特异性吸附及疏水性强的溶质的非特异性疏水性吸附，作为用于此目的方案之一，在液相色谱法用柱硬件(1)的金属制流动相液体接触部(3)层叠以SiO2为主成分的第一膜(41)，在第一膜(41)的表面(411)层叠将SiO2表面的硅烷醇基经烷基甲硅烷基化而成的第二膜(42)。