Adherence promoting matrix for primary culture of dental pulp stem cells and culture method of primary dental pulp stem cells

The invention provides an adherence promoting matrix for primary culture of dental pulp stem cells and a culture method of primary dental pulp stem cells. The invention provides a material capable ofpromoting cell adherence growth, and a culture mode of coating a culture dish with the material is used for establishing a mature in-vitro culture system for primary preparation of the dental pulp stem cells and providing high-quality and rich cell sources for subsequent experimental research. During primary culture of the dental pulp stem cells, polyphenol protein is used as an adherence promoting reagent to pre-coat the culture dish, so that the tissue block cell climbing rate and the number of primary cells are increased, the primary preparation time is shortened, and the success rate of primary culture is increased. 一种牙髓干细胞原代培养促贴壁基质及原代牙髓干细胞的培养方法，本发明提供了一种可促进细胞贴壁生长的材料，用其包被培养皿的培养方式，为牙髓干细胞原代制备建立成熟的体外培养体系并且为后续实验研究提供优质及丰富的细胞来源，本发明在牙髓干细胞原代培养时，使用多酚蛋白作为促贴壁试剂预包被培养皿，增加了组织块细胞爬出率及原代细胞的数量，缩短了原代制备时间，提升了原代培养的成功率。