Refining method of vitamin B12 and application of obtained product

The invention discloses a refining method of vitamin B12. The refining method of the vitamin B12 disclosed by the invention comprises the following steps of: mixing a vitamin B12 crude product with anorganic solvent, cooling to 0-5 DEG C, adding a reducing agent, performing stirring at room temperature for 1-2 hours, performing filtering, and performing vacuum drying at 40-60 DEG C to obtain a refined vitamin B12 pure product, wherein the purity of the vitamin B12 pure product is greater than 99%. The vitamin B12 is creatively used as an auxiliary drug of BNCT drugs in the invention; the enrichment effect of the BNCT drugs in tumour cells is more obvious through synergistic use; and meanwhile, the vitamin B12 also has the effects of protecting cells, improving the immunity of organisms and the like. 本发明公开了一种维生素B12的精制方法。本发明所述的维生素B12的精制方法：将维生素B12粗品与有机溶剂混合，降温至0～5℃，然后加入还原剂，在室温下搅拌1～2小时，过滤，在40～60℃下真空干燥，得到精制后的维生素B12纯品，所述的维生素B12纯品的纯度大于99％。本发明创造性的使用维生素B12作为BNCT类药物辅助药物，通过协同使用使得BNCT类药物在肿瘤细胞内的富集作用更加明显，同时维生素B12还有细胞保护，提高机体