Construction method of standardized umbilical cord blood hematopoietic stem cell bank
The invention discloses a construction method of a standardized umbilical cord blood hematopoietic stem cell bank. The construction method of the standardized umbilical cord blood hematopoietic stem cell bank comprises the following steps: extracting umbilical cord blood hematopoietic stem cells (CB...
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| creator | LIANG HAIYAN DU SHAOYIN LI SHIYUE WU ZHIJUN DING XIANFENG LIU HAIXIA ZHONG HUILIN ZOU QINGJIAN ZOU QINGYAN WANG BIN |
| description | The invention discloses a construction method of a standardized umbilical cord blood hematopoietic stem cell bank. The construction method of the standardized umbilical cord blood hematopoietic stem cell bank comprises the following steps: extracting umbilical cord blood hematopoietic stem cells (CBHSC) from umbilical cord blood by using a self-made stem cell separating medium, and performing ABO/Rh blood type, HLA typing and microbiological detection on the umbilical cord blood before extraction; carrying out related quality detection on the obtained CBHSC, treating the CBHSC meeting the quality standard by using a cryoprotectant, subpackaging the CBHSC into cryopreservation tubes or cryopreservation bags, carrying out gradient cooling, carrying out liquid nitrogen cryopreservation according to ABO/Rh blood types and HLA types, and establishing a CBHSC information file for retrieval, namely a standardized CBHSC library. Compared with a conventional umbilical cord blood bank, the standardized CBHSC bank const |
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The construction method of the standardized umbilical cord blood hematopoietic stem cell bank comprises the following steps: extracting umbilical cord blood hematopoietic stem cells (CBHSC) from umbilical cord blood by using a self-made stem cell separating medium, and performing ABO/Rh blood type, HLA typing and microbiological detection on the umbilical cord blood before extraction; carrying out related quality detection on the obtained CBHSC, treating the CBHSC meeting the quality standard by using a cryoprotectant, subpackaging the CBHSC into cryopreservation tubes or cryopreservation bags, carrying out gradient cooling, carrying out liquid nitrogen cryopreservation according to ABO/Rh blood types and HLA types, and establishing a CBHSC information file for retrieval, namely a standardized CBHSC library. 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The construction method of the standardized umbilical cord blood hematopoietic stem cell bank comprises the following steps: extracting umbilical cord blood hematopoietic stem cells (CBHSC) from umbilical cord blood by using a self-made stem cell separating medium, and performing ABO/Rh blood type, HLA typing and microbiological detection on the umbilical cord blood before extraction; carrying out related quality detection on the obtained CBHSC, treating the CBHSC meeting the quality standard by using a cryoprotectant, subpackaging the CBHSC into cryopreservation tubes or cryopreservation bags, carrying out gradient cooling, carrying out liquid nitrogen cryopreservation according to ABO/Rh blood types and HLA types, and establishing a CBHSC information file for retrieval, namely a standardized CBHSC library. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Construction method of standardized umbilical cord blood hematopoietic stem cell bank |
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