Construction method of standardized umbilical cord blood hematopoietic stem cell bank

The invention discloses a construction method of a standardized umbilical cord blood hematopoietic stem cell bank. The construction method of the standardized umbilical cord blood hematopoietic stem cell bank comprises the following steps: extracting umbilical cord blood hematopoietic stem cells (CBHSC) from umbilical cord blood by using a self-made stem cell separating medium, and performing ABO/Rh blood type, HLA typing and microbiological detection on the umbilical cord blood before extraction; carrying out related quality detection on the obtained CBHSC, treating the CBHSC meeting the quality standard by using a cryoprotectant, subpackaging the CBHSC into cryopreservation tubes or cryopreservation bags, carrying out gradient cooling, carrying out liquid nitrogen cryopreservation according to ABO/Rh blood types and HLA types, and establishing a CBHSC information file for retrieval, namely a standardized CBHSC library. Compared with a conventional umbilical cord blood bank, the standardized CBHSC bank const