Basophilic granulocyte degranulation detection kit, detection method and application thereof

The invention discloses a basophilic granulocyte degranulation detection kit, which comprises a stimulation buffer solution, a stimulation quality control A, a stimulation quality control B, a dyeingreagent, a dissolving reagent and a cleaning buffer solution; wherein the stimulation buffer solution comprises calcium ions, heparin and interleukin 3; the stimulation quality control A is a monoclonal antibody of an anti-high affinity IgE receptor; the stimulation quality control B is fMLP; the staining reagent is a mixture of anti-CD63-FITC and anti-CCR3-PE. The invention also discloses application of the kit and a detection method of the kit. The kit disclosed by the invention can be used for detecting the perioperative anaphylactic reaction, a new method is provided for improving the diagnosis and treatment of the perioperative anaphylactic reaction, so that the anaphylactic reaction is diagnosed in time, and the allergen is correctly recognized. 本发明公开了一种嗜碱性粒细胞脱颗粒检测试剂盒，包括刺激缓冲液、刺激质控A、刺激质控B、染色试剂、溶解试剂和清洗缓冲液；所述刺激