Method for determining content of hydrofossil B in achyranthes bidentata

The invention provides a method for determining the content of hydrofossil B in achyranthes bidentata and belongs to the technical field of analysis and detection of effective components of traditional Chinese medicines. The detection method comprises the following steps of respectively preparing a test solution and a reference solution, injecting the reference solution and the test solution intoa high performance liquid chromatograph, and eluting by taking an acetonitrile -0.1% formic acid aqueous solution as a mobile phase. The method is simple and convenient to operate, high in stability,good in repeatability and strong in specificity, and has the advantages of simple preparation of a test sample, low equipment requirement, safe experimental operation, high stability, good repeatability, strong specificity, simple and convenient operation and the like; a reliable detection method is provided for providing product quality control and evaluation indexes for follow-up qualitative andquantitative research and