High-specificity and high-sensitivity nucleic acid detection method based on modified dNTP

The invention discloses a high-specificity and high-sensitivity nucleic acid detection method based on modified dNTP. The high-specificity and high-sensitivity nucleic acid detection method comprisesan SEA-PCR DNA or RNA detection method and an SEA-ITA isothermal amplification DNA or RNA sample detection method. The SEA-PCR detection method and the SEA-ITA isothermal amplification detection method are established, and modified substrates dNTP alpha Se or dNTP alpha S are used, so that the generation of non-specific products in the detection process is inhibited, the specificity, sensitivity,interference resistance and accuracy of a reaction are improved, the design of primers and the optimization of experimental conditions are simplified, an efficient amplification reaction is established, the non-specific amplification of the detection system is inhibited through the improvement of specificity, and the detection sensitivity and accuracy are improved especially under the condition oflow-concentration nucleic