Method for screening gentamicin high-yield strains for breeding by applying genome rearrangement technology

The invention discloses a method for screening gentamicin high-yield strains for breeding by applying a genome rearrangement technology. The method specifically comprises the steps of breeding a parent strain for genome rearrangement, carrying out genome rearrangement and carrying out detection, wherein the genome rearrangement comprises the steps of protoplast preparation, protoplast ultravioletinactivation and thermal inactivation and the like. The method has obvious effects. The method can be used for screening excellent strains of micromonospora purpurea, improving the existing screeningmethod, improving the breeding speed and improving the titer and the fermentation yield of fermentation liquor. 本发明公开了一种应用基因组重排技术筛选庆大霉素高产菌株选育的方法，具体包括选育基因组重排的亲本菌株、基因组重排以及检测，其中基因组重排包括原生质体制备、原生质体紫外灭火和热灭活等步骤，本发明效果显著，可用于绛红色小单孢菌优良菌株的筛选，改善现有的筛选方法，加快选育速度，提高发酵液的效价、发酵产量。