Nucleic acid detection method combining DNA/Fe3O4 reticular structure with magnetic three-phase extraction method

The invention discloses a nucleic acid detection method combining a DNA/Fe3O4 reticular structure with a magnetic three-phase extraction method. The nucleic acid detection method comprises the following steps: (1) preparing Fe3O4 nanosheets; (2) modifying single-stranded DNA on the Fe3O4 nanosheets to obtain Fe3O4/ssDNA; (3) preparing a hyperbranched DNA structure sample, and mixing the hyperbranched DNA structure sample with Fe3O4/ssDNA for reaction to obtain a DNA/Fe3O4 reticular structure; and (4) taking the system of the DNA/Fe3O4 reticular structure as a water phase, mixing with an organic phase, immersing a magnetic rod with extraction liquid drops into the organic phase, extracting the DNA/Fe3O4 reticular structure, taking out, monitoring the liquid drops subjected to the catalyticchromogenic reaction by using an ultraviolet-visible spectrograph, and analyzing ultraviolet-visible spectral data to obtain the content of the target nucleic acid in a sample to be detected. According to the method, the dete