Colorimetric biosensor for detecting exosome based on Au@Ag

The invention provides a colorimetric biosensor for detecting exosome based on Au@Ag. The colorimetric biosensor comprises heme, Au@Ag, H2O2, aptamers EpCAM Apt and CD63 Apt, and hairpin probes H1, H2and H3, and the sequences of the above components are sequentially shown as SEQ ID No: 1-5. The sensor provided by the invention has the advantages of high detection speed, simple operation, low price, low detection limit, high specificity and the like, can make up for the defects and deficiencies of an exosome embodied detection method, and realizes rapid and accurate quantitative detection of the exosome. 本发明提供了一种基于Au@Ag检测外泌体的比色生物传感器，包括血红素、Au@Ag、H2O2和适配体EpCAM Apt和CD63 Apt，发卡探针H1、H2和H3，序列依次如SEQ ID No:1-5所示。本发明的传感器具有检测速度快、操作简单、价格低廉、检测限低、特异性高等优点，可以弥补外泌体现有检测方法的缺陷与不足，实现对其快速、准确的定量检测。