Protein A affinity chromatography medium based on PMMA matrix as well as preparation method and application of Protein A affinity chromatography medium

The invention relates to a Protein A affinity chromatography medium based on a PMMA matrix and a preparation method and application of the Protein A affinity chromatography medium. The preparation method comprises the following steps: carrying out hydrophilization treatment on polymethyl methacrylate microspheres to prepare hydrophilic poly(methyl methacrylate) microspheres; mixing the hydrophilicpoly(methyl methacrylate) microspheres, alkali and an epoxy compound, and conducting reacting to prepare epoxidized poly(methyl methacrylate) microspheres; and subjecting the epoxidized poly(methyl methacrylate) microspheres, Protein A, ethylenediamine tetraacetic acid, mercaptoglycerol and auxiliaries to mixing and reacting to prepare the Protein A affinity chromatography medium based on the PMMA matrix. The Protein A affinity chromatography medium has the advantages of being good in hydrophilicity, strong in alkali resistance and acid resistance, small in particle size, uniform in particlesize, high in mechanical s