Compound, preparation method and application of compound as ratio type fluorescent probe for detecting pH

The invention discloses a compound, a preparation method and application of the compound as a ratio type fluorescent probe for detecting pH, and the chemical structure of the compound is shown in thespecification. The compound can be used as a fluorescent probe for detecting pH in endoplasmic reticulum, has endoplasmic reticulum targeting ability, and can realize ratio fluorescence imaging to sensitively detect pH change of the endoplasmic reticulum. 本发明公开了化合物及制备方法与其作为检测pH比率型荧光探针的应用，化合物的化学结构为：该化合物可以作为内质网内pH的荧光探针，具有内质网靶向能力，且能够实现比率荧光成像灵敏检测内质网pH变化。