A system and method of interfering with target genes

The invention relates to the field of biotechnologies, and particularly provides a system and a method for interfering with target genes. The system for interfering with target genes provided by the invention comprises a dCpf1-PS fusion protein, a Donor vector with a PS transposase recognition seque...

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Bibliographische Detailangaben
Hauptverfasser:	LIU ZHIGUO, LIU YING, LI KUI, MOU YULIAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the field of biotechnologies, and particularly provides a system and a method for interfering with target genes. The system for interfering with target genes provided by the invention comprises a dCpf1-PS fusion protein, a Donor vector with a PS transposase recognition sequence pair, and a guide RNA. The system of the present invention can achieve accurate integration of transgenic sequences, insertion of exogenous genes or interference with the target genes. 本发明涉及生物技术领域，具体而言，提供了一种对目标基因进行干扰的系统和方法。本发明提供的对目标基因进行干扰的系统，包括dCpf1-PS融合蛋白、具有PS转座酶识别序列对的Donor载体，以及引导RNA。可以实现转基因序列的精确整合，实现外源基因的插入或目标基因的干扰。