RDA method and kit for rapidly detecting human adenoviruses

The invention discloses an RDA method and kit for rapidly detecting human adenovirus these. The kit comprises a specific primer pair and an RDA fluorescence labeling probe, so as to realize safe, specific, sensitive, simple and convenient detection of human adenoviruses (HAdV), and overcome the defects of the existing traditional detection technology. According to the kit provided by the invention, a nucleic acid extraction step can be omitted, the detection of the human adenoviruses can be realized at the constant temperature of 37-42 DEG C within 20 minutes, the specificity is 100%, and thekit is very suitable for on-site rapid detection. Compared with a common PCR method, the RDA fluorescence method has the advantages that the reaction is performed at constant temperature, the temperature change is not needed, complex instruments are not needed, and the reaction time is shorter. Therefore, the method and the kit provided by the invention have the characteristics of simplicity and rapidness in operation, go