Genetically engineered bacterium for producing muconic acid by taking phenolic compound as raw material

The invention discloses a genetically engineered bacterium for producing muconic acid by taking a phenolic compound as a raw material. The genetically engineered bacterium is a genetically engineeredbacterium obtained by knocking both a muconate cycloisomerase gene and a catechol 2, 3-dioxygenase gene in rhodococcus. According to the genetically engineered bacterium, a muconic acid degradation pathway in microorganisms is blocked, so that the genetically engineered bacterium uses the phenolic compound to produce the muconic acid with relatively high additional value, and various phenolic compounds, such as catechol, phenol and guaiacol, can be used; and on the basis of realizing green and efficient degradation of the phenolic compounds, the furoic acid with the high additional value is biosynthesized by utilizing wastewater. 本发明公开了一种以含酚类化合物为原料生产粘糠酸的基因工程菌。所述的基因工程菌为将红球菌中的粘糠酸环化异构酶基因和邻苯二酚2,3双加氧酶基因双敲除得到的基因工程菌。本发明通过将微生物体内的粘糠酸降解途径进行阻断，使其利用酚类化合物生产附加值较高的粘糠酸，且能够利用多种酚类化合物，如邻苯二酚、苯酚、愈创木酚等，在实现含酚类化合物绿色高效降解的基础上，利用废水生物合成具有高附加