Electrochemiluminescence immunoassay kit for novel coronavirus vaccine expressedantigen protein

The invention provides an electrochemiluminescence immunoassay kit for novel coronavirus vaccine expressed antigen protein. The kit at least comprises a streptavidin-coated pore plate, a biotin-labeled anti-novel-coronavirus spike protein antibody 1, an SULFO-labeled anti-novel-coronavirus spike pro...

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Hauptverfasser: MIAO JINGYUN, ZHANG XINGLIN, WANG HENGLING, GE PINGJU, JIAO QIULING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides an electrochemiluminescence immunoassay kit for novel coronavirus vaccine expressed antigen protein. The kit at least comprises a streptavidin-coated pore plate, a biotin-labeled anti-novel-coronavirus spike protein antibody 1, an SULFO-labeled anti-novel-coronavirus spike protein antibody 2, a washing solution, a reading solution, a novel coronavirus S protein standard substance and a novel coronavirus RBD protein standard substance. According to the invention, the biotin-labeled anti-novel-coronavirus spike protein antibody 1 is connected with the streptavidin plate tobe used as a stationary phase, and novel coronavirus S protein and RBD protein are used as reference substances and can be recognized by the SULFO-labeled antibody 2, so that the expression of a novel coronavirus antigen is detected. The kit can accurately, sensitively and quantitatively detect novel coronavirus S protein and RBD protein in different matrixes, the pretreatment process of a sampleis simple, consumed time