Tissue culture rapid propagation method for rieger begonia

The invention belongs to the technical field of plant tissue culture, and particularly relates to a tissue culture rapid propagation method for rieger begonia. According to the method, the top end ofa single bud of the rieger begonia serves as an explant, and a rieger begonia tissue culture rapid propagation technical system is established through the steps of explant disinfection, bud primary induction culture, subculture, rooting culture, seedling hardening, transplanting and the like, so that the purpose of rapid propagation of the rieger begonia is achieved. According to the method, low-temperature induction and subculture are adopted, risks of vitrification and browning of culture seedlings can be reduced, and the method is widely applied to the technical field of tissue culture rapid propagation of malus spectabilis. 本发明属于植物组织培养技术领域，具体地说，涉及一种丽格海棠组培快速繁殖方法；本发明以丽格海棠单芽顶端为外植体，经过外植体消毒、芽初代诱导培养、继代培养、生根培养、炼苗及移栽等步骤建立了丽格海棠组织培养快繁技术体系，从而实现丽格海棠快速繁殖的目的，本发明所采用的低温诱导和继代培养，降低了培养苗体的玻璃化和褐化的风险，本发明广泛应用于海棠组织培养快速繁殖技术领域。