Biotin labeled primer extension method and application thereof

The invention discloses a biotin labeled primer extension method and application thereof. According to the biotin labeled primer extension method, an oligonucleotide primer with a label is annealed and combined to a template RNA chain, free dNTPs extend under the action of reverse transcriptase to synthesize a cDNA complementary chain, and a reverse transcription product is separated and displayedthrough denatured gel electrophoresis and label tracing. The biotin labeled primer extension method is characterized in that the oligonucleotide primer with the label is an oligonucleotide primer with a biotin label. Experiment conditions are optimized, stable and harmless biotin labeling is used for replacing isotope labeling, a non-isotope labeled primer extension method is provided, the safetyproblem of an isotope method is solved, meanwhile, the detection precision is improved, and the experiment period is shortened. 本发明公开了一种生物素标记引物延伸方法及其应用。是使带有标记物的寡核苷酸引物退火结合到模板RNA链上，游离的dNTPs在反转录酶作用下延伸合成cDNA互补链，通过变性凝胶电泳及标记物示踪分离并展