Chemiluminiscence kit for detecting anti-nucleocapsid protein antibody in serum of HCV infected person, and detection method

The invention relates to the technical field of biology, and discloses a chemiluminiscence kit for detecting an anti-nucleocapsid protein (anti-Core) antibody in serum of an HCV infected person. The chemiluminiscence kit comprises a microwell plate obtained by coating with a recombinant HCV nucleocapsid protein antigen, and a sample diluent, a negative control, a positive control, an enzyme markerworking solution, a substrate solution A, a substrate solution B and a washing solution which are independently packaged, wherein the substrate solution A comprises luminol, o-phenylphenol, 4-imidazole phenol and a carbonic acid buffer solution (CB), and the substrate solution B comprises urea peroxide and a phosphate buffer solution (PB). According to the invention, the kit can be used for carrying out in-vitro qualitative/quantitative determination on the anti-Core antibody in the serum of an HCV infected person, establishing a detection technology of the anti-Core antibody in the serum ofthe HCV infected person an