Method for separating and purifying stable-ploidy regeneration plants from polyploidy dioscorea alata

The invention relates to a method for separating and purifying stable-ploidy regeneration plants from polyploidy dioscorea alata. The method mainly comprises the following steps of (1) sterilizing stems; (2) inducing calluses; (3) carrying out differentiation culture; (4) carrying out rooting culture; (5) carrying out root tip chromosome number identification; and (6) carrying out biological character and chromosome ploidy stability re-identification. The method is ideal in design, tissue culture is carried out on the polyploid (such as tetraploid and hexaploid) dioscorea alata stems serving as explants, the regeneration plants with single ploidy and stable biological characters can be obtained, strains with different chromosome ploidy and biological characters can also be obtained, and the polyploidy dioscorea alata can be used in a breeding plan. The germplasm resources of the dioscorea alata are enriched, the breeding time is shortened, a new way is opened up for dioscorea alata breeding, and a foundation