Method for differentiating embryonic stem cells into CD34+ hematopoietic progenitor cells

The invention provides an improved EB (embryoid body) differentiation system. The differentiation system can be used for differentiating an EB into CD34+ hematopoietic progenitor cells under the conditions of normoxia and no xenogeneic cells. The invention also provides a method for differentiation...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	GUO LEILEI, CHEN XIAOFENG, OU ZHENSHENG, LIANG DECAN, LI WENJIA, ZHANG SHIDU, LI JINGQIU, LIU JING, SONG YIZHE
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention provides an improved EB (embryoid body) differentiation system. The differentiation system can be used for differentiating an EB into CD34+ hematopoietic progenitor cells under the conditions of normoxia and no xenogeneic cells. The invention also provides a method for differentiation culture on the EB by the improved EB differentiation system. The efficient formation of the EB is realized, and the problem that EB differentiation balls are easy to adhere is solved. 本发明提供了一种改进的拟胚体(EB)分化系统，所述分化系统能够使拟胚体(EB)在常氧、无异种来源细胞的条件下向CD34+造血祖细胞的分化。本发明还提供了利用改进的拟胚体(EB)分化系统对所述拟胚体(EB)进行分化培养的方法，实现了拟胚体(EB)的高效形成，而且克服了拟胚体(EB)分化球易粘连的问题。