Rapid and accurate coat color trait fixing method for breeding new black pig variety and application
The invention provides a rapid and accurate coat color trait fixing method and a rapid breeding method for a new black pig variety. The rapid and accurate coat color trait fixing method comprises thefollowing steps: firstly, observing and counting male parent, female parent, F1 generation and F2 gen...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention provides a rapid and accurate coat color trait fixing method and a rapid breeding method for a new black pig variety. The rapid and accurate coat color trait fixing method comprises thefollowing steps: firstly, observing and counting male parent, female parent, F1 generation and F2 generation coat color phenotypes and proportions of the bred black pig variety to determine that coatcolor inheritance of the variety belongs to single-gene control; secondly, screening genes for controlling the coat color, and preliminarily determining a major gene (the major gene of black coat is an MC1R gene); then performing extraction, amplification and sequencing to obtain the genotype of the major gene (the F2 generation genotype black homozygote is EE, the heterozygote is EE and the black and white spotted pig is EE), and meanwhile, determining that the hair color of the cultivated variety is dominant heredity under single-gene control; and finally, breeding blackpigs with black homozygote (EE) genotypes reser |
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