Preparation method and application of burkholderia cenocepacia and metabolites thereof
The invention relates to the technical field of biology, and discloses a preparation method and application of burkholderia cenocepacia and metabolites thereof. Specifically, the invention provides burkholderia cenocepacia, and the preservation number of the burkholderia cenocepacia is GDMCC No.6105...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention relates to the technical field of biology, and discloses a preparation method and application of burkholderia cenocepacia and metabolites thereof. Specifically, the invention provides burkholderia cenocepacia, and the preservation number of the burkholderia cenocepacia is GDMCC No.61058. The method for preparing metabolites of the burkholderia cenocepacia comprises the following steps: activating burkholderia cenocepacia, mixing the activated burkholderia cenocepacia with a liquid fermentation medium, performing primary fermentation, mixing the fermented burkholderia cenocepaciawith macroporous resin, and performing secondary fermentation to obtain a fermentation broth; performing solid-liquid separation on the fermentation broth to obtain a solid mixture; and contacting thesolid mixture with an organic solvent, performing extraction to obtain an extracted solution, concentrating the extracted solution, performing centrifuge and filtration to obtain metabolites of the burkholderia cenocepacia. T |
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