ENZYMATIC ENRICHMENT OF DNA-PORE-POLYMERASE COMPLEXES

The present invention provides a method for isolating Sequencing complexes, said method comprising forming a complex between a nanopore covalently linked to a polymerase and an oligonucleotide that isassociated with a purification moiety, separating any unbound/uncomplexed nanopores and oligonucleotides from the complexes by use of a solid support capable of binding the purification moiety, and cleaving bound complexes from the solid support with an enzyme composition. 本发明提供一种用于分离测序复合物的方法，所述方法包括在与聚合酶共价连接的纳米孔和与纯化部分缔合的寡核苷酸之间形成复合物，使用能够结合所述纯化部分的固体支撑物从所述复合物分离出任何未结合/未复合的纳米孔和寡核苷酸，以及使用酶组合物从所述固体支撑物裂解结合的复合物。