Real-time fluorescent PCR detection primers, probe, method and kit for drug native plant Catha134 edulis and products thereof

The invention discloses real-time fluorescent PCR detection primers, probe, method and kit for Catha134 edulis and products thereof. According to the invention, the primers and the probe are designedfor a specific sequence of the chloroplast of the Catha134 edulis; the specific real-time fluorescent PCR detection method for the Catha134 edulis is established; the method has the advantages of goodspecificity, high sensitivity, good repeatability and the like; and the minimum detection lower limit is 0.02%. The method effectively solves the problem of a specific rapid detection technology of the Catha134 edulis, and can be applied to detection of the Catha134 edulis and the products thereof in entry and exit port inspection and quarantine supervision. 本发明公开了一种恰特草及其制品实时荧光PCR检测引物、探针、方法和试剂盒。本发明针对恰特草叶绿体特异性序列设计引物和探针，建立恰特草特异性实时荧光PCR检测方法，具有特异性好、灵敏度高、可重复性良好等优点，最低检测下限为0.02％。有效的解决恰特草特异性快速检测技术的问题，该方法可应用于进出境口岸检验检疫监管的恰特草及其产品的检测。