Method for determining content of tribulus terrestris saponin K in fried tribulus terrestris or tribulus terrestris

The invention discloses a method for determining the content of tribulus terrestris saponin K in fried tribulus terrestris or tribulus terrestris. The method comprises the following steps of: a test solution preparation step, a reference solution preparation step, and a detecting and analyzing step. The method detects the content of tribulus terrestris saponin K in fried tribulus terrestris or tribulus terrestris standard decoction and formula granules by using an ultra performance liquid chromatograph and evaporation light scattering detection (UPLC-ELSD) for the first time. The detection method is high in sensitivity, good in repeatability and high in recovery rate. The invention establishes a detection method taking tribulus terrestris saponin K as a detection index, and has important significance for quality control research of fried tribulus terrestris or tribulus terrestris standard decoction and formula granules. 本发明公开了一种炒蒺藜或蒺藜中蒺藜皂苷K含量的测定方法，包括供试品溶液制备、对照品溶液制备、检测和分析步骤，本发明首次利用超高效液相色谱仪和蒸发光散射检测(UPLC-ELSD)检测