Fluorescent ERA constant-temperature rapid detection kit for African swine fever viruses

The invention relates to a fluorescent ERA constant-temperature rapid detection kit for African swine fever viruses and a use method and application thereof. The fluorescent ERA constant-temperature rapid detection kit is developed by utilizing a recombinase ERA amplification technology. According to the fluorescent ERA constant-temperature rapid detection kit, a conserved region K205R gene of anAfrican swine fever gene sequence is used as a target gene, specific primers and probes are designed according to a primer and probe design principle, and through tests such as primer and probe pairing and screening tests, reaction condition optimization, specificity, sensitivity and simulated clinical sample verification, an accurate, sensitive, rapid, simple and convenient fluorescent ERA constant-temperature rapid detection method for the African swine fever viruses is established finally and the kit is assembled; and a fluorescent quantitative PCR instrument is utilized, a result can be judged only by reacting for